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Plant Virology Protocols From Virus Isolation to Transgenic Resistance

Aus der Reihe Methods in Molecular Biology
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138,99 € UVP 160,49 €

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

24.02.1998

Abbildungen

XV, 571 p. 111 illus.

Herausgeber

Gary D. Foster + weitere

Verlag

Humana Press

Seitenzahl

571

Maße (L/B/H)

23,4/15,6/3,2 cm

Gewicht

1001 g

Auflage

1998 edition

Sprache

Englisch

ISBN

978-0-89603-385-6

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

24.02.1998

Abbildungen

XV, 571 p. 111 illus.

Herausgeber

Verlag

Humana Press

Seitenzahl

571

Maße (L/B/H)

23,4/15,6/3,2 cm

Gewicht

1001 g

Auflage

1998 edition

Sprache

Englisch

ISBN

978-0-89603-385-6

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Plant Virology Protocols
  • Produktbild: Plant Virology Protocols
  • to Plant Virology.- to Classical Crossprotection.- History of Coat Protein-Mediated Protection.- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes.- Geminivirus Isolation and DNA Extraction.- Caulimovirus Isolation and DNA Extraction.- Reovirus Isolation and RNA Extraction.- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction.- Hordeivirus Isolation and RNA Extraction.- Furovirus Isolation and RNA Extraction.- Tobravirus Isolation and RNA Extraction.- Tobamovirus Isolation and RNA Extraction.- Potexvirus Isolation and RNA Extraction.- Carlavirus Isolation and RNA Extraction.- Potyvirus Isolation and RNA Extraction.- Trichovirus Isolation and RNA Extraction.- Ilarvirus Isolation and RNA Extraction.- Bromovirus Isolation and RNA Extraction.- Cucumovirus Isolation and RNA Extraction.- Nepovirus Isolation and RNA Extraction.- Comovirus Isolation and RNA Extraction.- Carmovirus Isolation and RNA Extraction.- Tymovirus Isolation and Genomic RNA Extraction.- Tombusvirus Isolation and RNA Extraction.- Luteovirus Isolation and RNA Extraction.- Cloning of Coat Protein Genes.- RNA Analysis.- RNA Fractionation by Density Gradient Centrifugation.- cDNA Library Construction for the Lambda ZAP®-Based Vectors.- PCR Cloning of Coat Protein Genes.- Antibody Production.- Expression Library Screening.- In Vitro Transcription and Translation.- Analysis of Coat Protein Expression Cassettes in Protoplasts.- DNA Sequencing.- Computer Analysis of Amino Acid Sequences.- Plant Transformation and Primary Analysis.- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation.- Potato Transformation.- Transformation of Tomato.- Tobacco Transformation.- Genetic Transformation of Wheat.-Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309).- Molecular Analysis of Transgenic Rice.- PCR Analysis of Transgenic Tobacco Plants.- Southern Analysis of Transgenic Tobacco Plants.- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes.- Western Analysis of Transgenic Plants.- Evaluation of Resistance.- Assaying Levels of Plant Virus by ELISA.- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization.- Detection and Quantification of Plant Viruses by PCR.- Assaying Levels of Virus with Local Lesion Hosts.- Field Testing Resistance of Transgenic Plants.- Agronomic Performance of Transgenic Plants.- Mechanisms of Resistance.- Mechanisms of Resistance.- Mechanisms of RNA-Mediated Resistance to Plant Viruses.- Detection of Risks Associated with Coat Protein Transgenics.- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.