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E. coli Plasmid Vectors Methods and Applications

Aus der Reihe Methods in Molecular Biology
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112,99 € UVP 128,39 €

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Nicola Casali + weitere

Verlag

Humana Press

Seitenzahl

316

Maße (L/B/H)

23,5/15,5/1,9 cm

Gewicht

505 g

Auflage

Softcover reprint of hardcover 1st ed. 2003

Sprache

Englisch

ISBN

978-1-61737-391-6

Beschreibung

Rezension

...a scientific review and a methodological "cook-book" of high quality. It is truly a highly practical handbook, useful for all scientists working with gene cloning and manipulation." - Folia Microbiologica

"...a very useful addition to laboratories investigating plasmids per se, and for those employing plasmids as vectors for gene cloning." - Microbiology Today

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Verlag

Humana Press

Seitenzahl

316

Maße (L/B/H)

23,5/15,5/1,9 cm

Gewicht

505 g

Auflage

Softcover reprint of hardcover 1st ed. 2003

Sprache

Englisch

ISBN

978-1-61737-391-6

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: E. coli Plasmid Vectors
  • Produktbild: E. coli Plasmid Vectors
  • The Function and Organization of Plasmids
    Finbarr Hayes

    Choosing a Cloning Vector
    Andrew Preston

    Escherichia coli Host Strains
    Nicola Casali

    Chemical Transformation of E. coli
    W. Edward Swords

    Electroporation of E. coli
    Claire A. Woodall

    DNA Transfer by Bacterial Conjugation
    Claire A. Woodall

    Cosmid Packaging and Infection of E. coli
    Mallory J. A. White and Wade A. Nichols

    Isolation of Plasmids from E. coli by Alkaline Lysis
    Sabine Ehrt and Dirk Schnappinger

    Isolation of Plasmids from E. coli by Boiling Lysis
    Sabine Ehrt and Dirk Schnappinger

    High-Purity Plasmid Isolation Using Silica Oxide
    Stefan Grimm and Frank Voß-Neudecker

    High-Throughput Plasmid Extraction Using Microtiter Plates
    Michael A. Quail

    Isolation of Cosmid and BAC DNA from E. coli
    Daniel Sinnett and Alexandre Montpetit

    Preparation of Single-Stranded DNA from Phagemid Vectors
    W. Edward Swords

    Using Desktop Cloning Software to Plan, Track, and Evaluate Cloning Projects
    Robert H. Gross

    Cloning in Plasmid Vectors
    Carey Pashley and Sharon Kendall

    Extraction of DNA from Agarose Gels
    Nicholas Downey

    Cloning PCR Products with T-Vectors
    Wade A. Nichols

    Construction of Genomic Libraries in l-Vectors
    Yilun Wang, Zheng Cao, Darryl Hood, and James G. Townsel

    Rapid Screening of Recombinant Plasmids
    Sangwei Lu

    Restriction Analysis of Recombinant Plasmids
    Joanne Goranson-Siekierke and Jarrod L. Erbe

    Screening Recombinant DNA Libraries
    Wade A. Nichols

    Sequencing Using Fluorescent-Labeled Nucleotides
    Allison F. Gillaspy

    Site-Directed Mutagenesis Using the Megaprimer Method
    Zhidong Xu, Alessia Colosimo, and Dieter C. Gruenert

    Site-Directed Mutagenesis byInverse PCR
    Clifford N. Dominy and David W. Andrews

    Creating Nested DNA Deletions Using Exonuclease III
    Rosamund Powles and Lafras M. Steyn

    Transposon and Transposome Mutagenesis of Plasmids, Cosmids, and BACs
    Alistair McGregor

    In Vitro Transcription and Translation
    Farahnaz Movahedzadeh, Susana González Rico, and Robert A. Cox

    Vectors for the Expression of Recombinant Proteins in E. coli
    Sally A. Cantrell

    Expression of Recombinant Proteins from lac Promoters
    Charles R. Sweet

    Plasmid-Based Reporter Genes: Assays for b-Galactosidase and Alkaline Phosphatase Activities
    Minghsun Liu

    Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein
    Sergei R. Doulatov

    Index