Part I: Maintenance and Cryopreservation of ES and iPS cells
1. Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells
Dong-Youn Hwang
2. Growth of Human Embryonic Stem Cells in Long-Term Hypoxia
Vladimir Zachar, Simon C. Weli, Mayuri S. Prasad, and Trine Fink
3. Laboratory–Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein that Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells
Masato Nagaoka and Stephen A. Duncan
4. Scale-Up of Single Cell-Inoculated Suspension Cultures of Human Embryonic Stem Cells
Harmeet Singh, Pamela Mok, and Robert Zweigerdt
5. Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells
Guang-wei Sun, Xiao-xi Xu, Nan Li, Ying Zhang, and Xiao-jun Ma
6. Expansion of Pluripotent Stem Cells in Defined Xeno-Free Culture System
Kristiina Rajala
7. Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells
Behrouz Aflatoonian and Harry D. Moore
8. Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Frida Holm
Part II: Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming
9. Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2
Alessandra Giorgetti, Nuria Montserrat, and Juan Carlos Izpisua Belmonte
10. Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood
Naoki Nishishita, Chiemi Takenaka, and Shin Kawamata
11. Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage
Maria V. Shutova, Ilya V. Chestkov, Maria A. Lagarkova, and Sergey L. Kiselev
12. Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells
Hua Liu, Su Mi Choi, and Yoon-Young Jang
13. Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders
Kazutoshi Takahashi
14. Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods)
Hiroe Ohnishi, Yasuaki Oda, and Hajime Ohgushi
15. Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes
Athanasia D. Panopoulos, Sergio Ruiz, and Juan Carlos Izpisua Belmonte
16. Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles
Ann Peters and Elias T. Zambidis
Part III: Generation of Patient-Specific iPS Cells for Clinical Application
17. Generation of iPS Cells from Human Skin Biopsy
Katie Avery and Stuart Avery
18. Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
Masashi Toyoda, Shogo Nagata, Hatsune Makino, Hidenori Akutsu, Takashi Tada, and Akihiro Umezawa
Part IV: Lineage-Specific Differentiation of hES and iPS Cells
19. In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells
Xiaolong Lin, Hua Jiang, Zack Zhengyu Wang, and Tong Chen
20. Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells
Kumiko Saeki
21. Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endothelial Cells under Hypoxic Condition
Xiuli Wang
22. Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Shijun Hu, Preston Lavinghousez, Zongjin Li, and Joseph C. Wu
23. Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture with Murine Stromal Cells
Feng Ma, Yanzheng Gu, Natsumi Nishihama, Wenyu Yang, Ebihara Yasuhiro, and Kohichiro Tsuji
24. Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells
Tea Soon Park, Paul W. Burridge, and Elias T. Zambidi
25. Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Michio Noguchi, Masakatsu Sone, Daisuke Taura, Kiminori Hosoda, and Kazuwa Nakao
26. Chondrogenic Differentiation of hESC in Micromass Culture
Deborah Ferrari, Guochun Gong, Robert A. Kosher, and Caroline N. Dealy
27. Deriving Hepatic Endoderm from Pluripotent Stem Cells
Claire N. Medine, Zara Hannoun, Sebastian Greenhough, Catherine M. Payne, Judy Fletcher, and David C. Hay
28. Multistage Hepatic Differentiation from Human Induced Pluripotent Stem Cells
Su Mi Choi, Hua Liu, Yonghak Kim, and Yoon-Young Jang
29. Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers
Takamichi Ishii and Kentaro Yasuchika
30. Generation of Lung Epithelial-Like Tissue from hESC by Air-Liquid Interface Culture
Lindsey Van Haute, Gert De Block, Inge Liebaers, Karen Sermon, and Martine De Rycke
31. Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groove Pattern Arrays
Kye-Seong Kim, Hosup Jung, and Keesung Kim
Part V: Directed Differentiation of hES and iPS Cells in 3D Environments
32. Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and MatrigelTM Gels
Eric Derby, Dezhong Yin, Wei-Qiang Gao, and Wu Ma
Part VI: Qualitative and Quantitative Analysis of Dynamics of hES Cell Differentiation
33. Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells
Jason D. Gibson, Caroline M. Jakuba, Craig E. Nelson, and Mark G. Carter
34. Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells
Agnete Kirkeby, Malin Parmar, and Johan Jakobsson
