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Band 681

Protein Chromatography Methods and Protocols

Aus der Reihe Methods in Molecular Biology

105,99 €

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

X, 527 p.

Herausgeber

Dermot Walls + weitere

Verlag

Humana Press

Seitenzahl

527

Maße (L/B/H)

25,4/17,8/2,9 cm

Gewicht

1003 g

Auflage

Softcover reprint of the original 1st ed. 2011

Sprache

Englisch

ISBN

978-1-4939-6129-0

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

23.08.2016

Abbildungen

X, 527 p.

Herausgeber

Verlag

Humana Press

Seitenzahl

527

Maße (L/B/H)

25,4/17,8/2,9 cm

Gewicht

1003 g

Auflage

Softcover reprint of the original 1st ed. 2011

Sprache

Englisch

ISBN

978-1-4939-6129-0

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Protein Chromatography
  • Produktbild: Protein Chromatography
  • A Digest of Protein Purification.- Gel-Filtration Chromatography.- Immunoaffinity Chromatography.- Avoiding Proteolysis During Protein Chromatography.- Scale-Up of Protein Purification: Downstream Processing Issues.- Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources.- Engineering Protein Stability.- Microfluidics in Protein Chromatography.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Storage and Lyophilization of Pure Proteins.- Differential Precipitation and Solubilization of Proteins.- Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase.- Protein Quantitation and Analysis of Purity.- Purification of Proteins Fused to Glutathione S-Tranferase.- Purification of Proteins Fused to Maltose-Binding Protein.- Purification of Proteins from Baculovirus-Infected Insect Cells.- Purification of Poly-Histidine-Tagged Proteins.- Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry.- Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins.- Purification of Antibodies Using Affinity Chromatography.- Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens.- Measuring Protein-Protein Interactions Using Biacore.- Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins.- Hydrophobic Interaction Chromatography.- Fast Protein Liquid Chromatography.- Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS.- Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Strategies for the Purification of Membrane Proteins.- A Multi-Step ChromatographicStrategy to Purify Three Fungal Endo-β-Glucanases.