Band 14
Acute immune response of healthy horses to linear DNA encoding Interleukin 12 and Interleukin 18 complexed with SAINT-18
Aus der Reihe
Wissenschaftliche Reihe der Klinik für Pferde
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- Taschenbuch
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Form:Einzelkauf Download
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Sprache:Englisch
23,70 €
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Produktdetails
Format
Kopierschutz
Nein
Family Sharing
Ja
Text-to-Speech
Nein
Erscheinungsdatum
20.04.2015
Verlag
Cuvillier Verlag eBooksSeitenzahl
202 (Printausgabe)
Dateigröße
4411 KB
Sprache
Englisch
EAN
9783736949799
The aim of this study was to investigate the mechanisms exerted by complexed linear DNA vectors encoding Interleukin (IL-) 12 and IL-18 applied in vivo in horses. To enable this first suitable biomarkers for the acute immune response of horses were evaluated.
Within these, 15 monoclonal antibodies for cytokines of different species were evaluated for reactivity with equine cytokines. To accurately employ the cytokines and further selected parameters as biomarkers for treatment effects, internal controls were included and evaluated for influencing physiological factors. The age, sex and type of the horses influenced leukocyte composition, cell morphology and cytokine release by peripheral blood mononuclear cells (PBMC) ex vivo.
Linear DNA vectors encoding equine IL-12/-18 complexed with transfection reagent (SAINT-18) or comparative substances (SAINT-18 only, complexed nonsense DNA, complexed nonsense DNA without CpG) were applied to 24 healthy horses. All groups receiving complexed linear DNA vectors showed a systemic and local inflammatory response. This appeared basically independent of expression and CpG content of the DNA applied. Moreover, expressions of IL-12 and IL-18 enhanced immunostimulation and induced Th1 downstream mediators. Grey horses showed significantly fewer effects of DNA-treatments and were classified non-responders to immunostimulatory effects of linear DNA complexed with SAINT-18.
Within these, 15 monoclonal antibodies for cytokines of different species were evaluated for reactivity with equine cytokines. To accurately employ the cytokines and further selected parameters as biomarkers for treatment effects, internal controls were included and evaluated for influencing physiological factors. The age, sex and type of the horses influenced leukocyte composition, cell morphology and cytokine release by peripheral blood mononuclear cells (PBMC) ex vivo.
Linear DNA vectors encoding equine IL-12/-18 complexed with transfection reagent (SAINT-18) or comparative substances (SAINT-18 only, complexed nonsense DNA, complexed nonsense DNA without CpG) were applied to 24 healthy horses. All groups receiving complexed linear DNA vectors showed a systemic and local inflammatory response. This appeared basically independent of expression and CpG content of the DNA applied. Moreover, expressions of IL-12 and IL-18 enhanced immunostimulation and induced Th1 downstream mediators. Grey horses showed significantly fewer effects of DNA-treatments and were classified non-responders to immunostimulatory effects of linear DNA complexed with SAINT-18.
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