Produktbild: Challenges of the Unseen World
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Challenges of the Unseen World A Laboratory Course in Microbiology

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73,99 € UVP 83,90 €

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

01.04.2018

Verlag

John Wiley & Sons

Seitenzahl

203

Maße (L/B/H)

27,4/21,1/1,3 cm

Gewicht

590 g

Auflage

1. Auflage

Sprache

Englisch

ISBN

978-1-55581-992-7

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

01.04.2018

Verlag

John Wiley & Sons

Seitenzahl

203

Maße (L/B/H)

27,4/21,1/1,3 cm

Gewicht

590 g

Auflage

1. Auflage

Sprache

Englisch

ISBN

978-1-55581-992-7

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Challenges of the Unseen World
  • Preface
     
    About the Authors
     
    Introduction
     
    The scientific method
     
    Experimental design
     
    Big data
     
    Documentation
     
    Safety
     
    Student Laboratory Safety Contract
     
    Appendix
     
    Challenge One: Identifying the bacteria causing infections in hospital patients
     
    Lab One
     
    Background
     
    Diversity and pure cultures
     
    Bright field and phase contrast microscopy
     
    Learning outcomes
     
    Objectives
     
    Part 1: Isolate bacteria from a mixed culture: Procedure: Streaking for isolated colonies
     
    Part 2: Examine bacterial cells under the microscope
     
    Procedure: Making a wet mount
     
    Procedure: Using the microscope
     
    Preparation for next lab
     
    Questions
     
    Lab Two
     
    Background
     
    Colony morphology and optimum temperature for growth
     
    Cell shape and bacterial spores
     
    The cell envelope
     
    Learning outcomes
     
    Part 1: Describe the colony morphology of the unknown
     
    Part 2: Describe the characteristics of an individual cell viewed under themicroscope
     
    Part 3: Determine the optimum temperature for growth
     
    Part 4: Determine if the unidentified microorganism is Gram-positive or Gram-negative.
     
    Procedure: Doing a Gram stain
     
    Preparation for next lab
     
    Questions
     
    Lab Three
     
    Background
     
    Modes of energy generation in bacteria
     
    Learning outcomes
     
    Part 1: Can the unidentified microorganism grow in the presence of bile salts and ferment lactose?
     
    Procedure: Streaking cells on MacConkey-lactose plates
     
    Part 2: Can the unidentified microorganism ferment glucose?
     
    Procedure: Glucose fermentation test
     
    Part 3: Does the unidentified microorganism use cytochrome C duringrespiration (Gram-negative bacteria)?
     
    Procedure: Oxidase test
     
    Part 4: Does the microorganism make catalase (Gram-positive bacteria)?
     
    Procedure: Catalase test
     
    Part 5: Is the microorganism motile?
     
    Procedure: Soft agar motility assay
     
    Questions
     
    Solving Challenge One
     
    Preparing for Challenge Two
     
    Questions
     
    Bibliography
     
    Challenge Two: Confirming the identification of a microorganism by sequencing the 16S rRNA gene
     
    Questions before you begin the challenge
     
    Lab One: Background
     
    Classification of bacteria and 16S rRNA gene
     
    Polymerase chain reaction (PCR)
     
    Lab One: Learning outcomes
     
    Lab One: Objective
     
    Part 1: Obtain enough DNA for sequencing: amplify the 16S rRNA gene by PCR
     
    Procedure:Diluting from stock solutions:
     
    Lab One: Questions
     
    Lab Two: Background
     
    Agarose gel electrophoresis
     
    Dideoxy DNA sequencing
     
    Lab Two: Learning outcomes
     
    Lab Two: Objectives
     
    Part 1: Visualize the PCR product by agarose gel electrophoresis
     
    Procedure:Making an agarose gel and carrying out gel electrophoresis
     
    Part 2: Submit sample for DNA sequencing
     
    Lab Two: Questions
     
    Solving Challenge Two: Background
     
    Solving Challenge Two: Learning outcomes
     
    Solving Challenge Two: Objective
     
    Identifying the unknown microorganism from the 16S rRNA gene sequence
     
    Procedure: Preparing the sequence for analysis
     
    Procedure: Doing a BLAST search
     
    Questions
     
    Bibliography
     
    Challenge Three: Choosing an antibiotic to alleviate the symptoms of Crohn's disease
     
    Questions before you begin the challenge