1. Introduction to FOXO Biology
Wolfgang Link
Part I: Biochemical and Molecular Methods
2. Characterization of microRNAs Regulating FOXO Expression
Doug Hanniford and Eva Hernando
3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter
Christopher P. Cardozo
4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover
Thomas Heimbucher and Coleen T. Murphy
5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity
Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco
6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes
María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos
7. Characterization of FOXO Acetylation
Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam
8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays
Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida
9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes
Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira
10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters
Neeraj Kumar and Arnab Mukhopadhyay
11. RNAi Mediated Silencing of FoxO Factors
Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas
Part II: Imaging Approaches
12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO
Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain
13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO
Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos
14. Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus
Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link
15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology
Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato
Part III: Omics and Bioinformatic Approaches
16. Genome-wide Analysis for Identifying FOXO Protein Binding Sites
Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang
17. Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors
Bradford E. Peercy and Martin F. Schneider
18. Following Transcriptome to Uncover FOXO Biological Functions
Raymond Liang, Vijay Menon, and Saghi Ghaffari
Part IV: Animal Models
19. Hydra as Model to Determine the Role of FoxO in Longevity
Thomas C. G. Bosch
20. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role
Hwanhee Oh and Jihye Paik
Part V: Human Gentic and Clinical Studies
21. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity
Timothy A Donlon, Philip MC Davy, and Bradley J Willcox
22. Analysis of FOXO3a Gene Polymorphism Associated with Asthma
Tejas Shah and Kalpana Joshi
