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Produktbild: Macrophage Migration Inhibitory Factor
Band 3002 - 11%

Macrophage Migration Inhibitory Factor Methods and Protocols

Aus der Reihe Methods in Molecular Biology
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189,99 € UVP 213,99 €

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.05.2026

Abbildungen

XII, 93 illus., 83 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

James Harris + weitere

Verlag

Springer Us

Seitenzahl

296

Maße (L/B/H)

26/18,3/2,3 cm

Gewicht

778 g

Auflage

Second Edition 2026

Sprache

Englisch

ISBN

978-1-07-165055-4

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

15.05.2026

Abbildungen

XII, 93 illus., 83 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Verlag

Springer Us

Seitenzahl

296

Maße (L/B/H)

26/18,3/2,3 cm

Gewicht

778 g

Auflage

Second Edition 2026

Sprache

Englisch

ISBN

978-1-07-165055-4

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Macrophage Migration Inhibitory Factor

  • Expression and Purification of Recombinant Macrophage Migration Inhibitory Factor (MIF).- Generation of MIF Peptides via Solid Phase Peptide Synthesis.- Expression and Purification of Recombinant D-Dopachrome Tautomerase (D-DT).- Structural Determination and Ligand Interaction of Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography Approach.- Generation of High-Quality D-Dopachrome Tautomerase Crystals for Structural Studies.- Fragment Screening of MIF by Surface Plasmon Resonance.- Detection of Noncovalent Protein-Ligand Complexes of D-DT Variants by MALDI-TOF Mass Spectrometry.- Analysis of D-Dopachrome Tautomerase Conformational Flexibility Through Molecular Dynamics Solutions.- MIF NMR Chemical Shift Perturbation Mapping.- Assessing the Tautomerase Activity of MIF Using L-Dopachrome.- Using 4-HPP and PP as Substrates to Assess Purified MIF and D-DT Tautomerase Activity and Inhibition.- Protocols for Assessing the Nuclease Activity of MIF.- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Biological Samples Using Enzyme-Linked Immunosorbent Assay (ELISA).- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Tissue Samples Using Immunohistochemistry (IHC).- Exploring the Chemotactic Effects of MIF on Neutrophils and T Cells and Methods for Selective Receptor Targeting.- Polarization of Bone Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing CATT7 Polymorphic Mice.- Using Luciferase Reporter Cells to Assess the Effects of MIF Inhibitors on NF-kB Activation.- Monitoring Inflammasome Activity through ASC Speck Formation in THP-1 ASC-GFP Cells.- Small Interfering RNA (siRNA)-Mediated Knockdown of Macrophage Migration Inhibitory Factor Transcript in Adherent and Suspension Cultures of Human Cell Lines.- Stable Transfection of CD74 Gene Variants into A549 Lung Adenocarcinoma Cell Line.- Methods to Determine the Role of MIF in Cilia Biogenesis.- Modelling Fungal and Amoebic Infections: Co-Culture Approaches to Study MIF’s Role in Infection Induced Inflammatory Responses.- Immunofluorescent Quantification of MIF in Larval Zebrafish Tailfin Transfection.