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Band 681

Protein Chromatography Methods and Protocols

Aus der Reihe Methods in Molecular Biology

138,99 €

inkl. gesetzl. MwSt., Versandkostenfrei

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.11.2010

Herausgeber

Dermot Walls + weitere

Verlag

Humana Press

Seitenzahl

527

Maße (L/B/H)

26,2/18,7/4,1 cm

Gewicht

1156 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-60761-912-3

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.11.2010

Herausgeber

Verlag

Humana Press

Seitenzahl

527

Maße (L/B/H)

26,2/18,7/4,1 cm

Gewicht

1156 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-60761-912-3

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Protein Chromatography
  • Produktbild: Protein Chromatography
  • Part I: Overviews

    1. A Digest of Protein Purification
    Dermot Walls, Robert McGrath, and Sinéad T. Loughran

    2. Gel-Filtration Chromatography
    Ciarán Ó’Fágáin, Philip M. Cummins, and Brendan F. O’Connor

    3. Immunoaffinity Chromatography
    Jenny Fitzgerald, Paul Leonard, Elaine Darcy, and Richard O’Kennedy

    4. Avoiding Proteolysis During Protein Chromatography
    Barry J. Ryan

    5. Scale-Up of Protein Purification: Downstream Processing Issues
    John Joseph Milne

    6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources
    William J.J. Finlay, Laird Bloom, and Orla Cunningham

    7. Engineering Protein Stability
    Ciarán Ó’Fágáin

    8. Microfluidics in Protein Chromatography
    Frank A. Gomez

    9. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification
    Dermot Walls and Sinéad T. Loughran

    Part II: Protocols

    10. Storage and Lyophilization of Pure Proteins
    Ciarán Ó’Fágáin

    11. Differential Precipitation and Solubilization of Proteins
    Barry J. Ryan

    12. Ion-Exchange Chromatography: Basic Principles and Application to the Partial Purification of Soluble Mammalian Prolyl Oligopeptidase
    Philip M. Cumminsa, Oonagh Dowling, and Brendan F. O’Connor

    13. Protein Quantitation and Analysis of Purity
    Eva M. Campion, Sinéad T. Loughran, and Dermot Walls

    14. Purification of Proteins Fused to Glutathione S-Tranferase
    Sandra Harper and David W. Speicher

    15. Purification of Proteins Fused to Maltose-Binding Protein
    Mario Lebendiker and Tsafi Danieli

    16. Purification of Proteins from Baculovirus-Infected Insect Cells
    Luke O’Shaughnessy and Sean Doyle

    17. Purification of Poly-Histidine-Tagged Proteins
    Sinéad T. Loughran and Dermot Walls

    18. Immobilized Metal Affinity Chromatography/Reversed-Phase Enrichment of Phosphopeptides and Analysis by CID/ETD Tandem Mass Spectrometry
    Rosana Navajas, Alberto Paradela, and Juan Pablo Albar

    19. Tag Removal by Site-Specific Cleavage of Recombinant Fusion Proteins
    Adam Charlton and Michael Zachariou

    20. Purification of Antibodies Using Affinity Chromatography
    Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, and Richard O’ Kennedy

    21. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens
    William J.J. Finlay, Laird Bloom, and Orla Cunningham

    22. Measuring Protein-Protein Interactions Using Biacore
    Paul Leonard, Stephen Hearty, and Richard O’Kennedy

    23. Ultra-Performance Liquid Chromatography-Mass Spectrometry of Proteins
    Timothy R. Croley

    24. Hydrophobic Interaction Chromatography
    Philip Cummins and Brendan O’Connor

    25. Fast Protein Liquid Chromatography
    Ashkan Madadlou, Siobhan O’Sullivan, and David Sheehan

    26. Shotgun Proteomics: A Qualitative Approach Applying Isoelectric Focusing on Immobilized pH Gradient and LC-MS/MS
    Laurent Geiser, Ali R. Vaezzadeh, Jacques M. P. Deshusses, and Denis F. Hochstrasser

    27. Shotgun Proteomics: A Relative Quantitative Approach Using Off-Gel Electrophoresis and LC-MS/MS
    Laurent Geiser, Loïc Dayon, Ali R. Vaezzadeh, and Denis F. Hochstrasser

    28. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems
    Michael Henry and Paula Meleady

    29. Strategies for the Purification of Membrane Proteins
    Sinead Marian Smith

    30. A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases
    Tracey McCarthy and Maria G. Tuohy